Journal
MOLECULAR CANCER
Volume 20, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s12943-021-01395-7
Keywords
Circular RNA; RNF220; miR-30a; Acute myeloid leukemia
Categories
Funding
- National Natural Science Foundation of China [81600119]
- Science and Technology Planning Project of Guangdong Province [2017A020215006]
- Guangzhou Women and Children's Medical Center/Guangzhou Institute of Pediatrics [YIP-2016-018]
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The circRNA circRNF220 showed high sensitivity and specificity in distinguishing pediatric AML, serving as a potential biomarker for accurate diagnosis and relapse prediction. Its function in inhibiting proliferation and promoting apoptosis in AML cells highlights its therapeutic potential. The mechanistic role of circRNF220 as a miR-30a sponge to regulate target genes MYSM1 and IER2 provides insights into AML pathogenesis and relapse mechanisms.
Background Circular RNAs (circRNAs) constitute a family of transcripts with unique structures and have been confirmed to be critical in tumorigenesis and to be potential biomarkers or therapeutic targets. However, only a few circRNAs have been functionally characterized in pediatric acute myeloid leukemia (AML). Methods Here, we investigated the expression pattern of circRNAs in pediatric AML using a circRNA microarray. The characteristics, potential diagnostic value, and prognostic significance of circRNF220 were evaluated. A series of functional experiments were performed to investigate the role of circRNF220 in primary pediatric AML cells. Then we investigated the aberrant transcriptional networks regulated by circRNF220 in primary AML cells by RNA-seq. Furthermore, biotin RNA pulldown assays were implemented to verify the relationship between circRNF220 and miR-30a. Results We identified a circRNA, circRNF220, which was specifically abundant in and accumulated in the peripheral blood and bone marrow of pediatric patients with AML. It could distinguish AML from ALL and other hematological malignancies with high sensitivity and specificity. Significantly, circRNF220 expression independently predicted prognosis, while high expression of circRNF220 was an unfavorable prognostic marker for relapse. Furthermore, we characterized the function of circRNF220 and found that circRNF220 knockdown specifically inhibited proliferation and promoted apoptosis in AML cell lines and primary cells. Mechanistically, circRNF220 may act as an endogenous sponge of miR-30a to sequester miR-30a and inhibit its activity, which increases the expression of its targets MYSM1 and IER2 and implicated in AML relapse. Conclusions Collectively, these findings demonstrated that circRNF220 could be highly efficient and specific for the accurate diagnosis of pediatric AML, with implications for relapse prediction.
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