An Insight Into Detection Pathways/Biosensors of Highly Infectious Coronaviruses

The outbreak of COVID-19 pandemic and its consequences have inflicted a substantial damage on the world. In this study, it was attempted to review the recent coronaviruses appeared among the human being and their epidemic/pandemic spread throughout the world. Currently, there is an inevitable need for the establishment of a quick and easily available biosensor for tracing COVID-19 in all countries. It has been known that the incubation time of COVID-19 lasts about 14 days and 25% of the infected individuals are asymptomatic. To improve the ability to determine SARS-CoV-2 precisely and reduce the risk of eliciting false-negative results produced by mutating nature of coronaviruses, many researchers have established a real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay using mismatch-tolerant molecular beacons as multiplex real-time RT-PCR to distinguish between pathogenic and non-pathogenic strains of coronaviruses. The possible mechanisms and pathways for the detection of coronaviruses by biosensors have been reviewed in this study.

Automated summary

The outbreak of COVID-19 pandemic has caused significant damage to the world, highlighting the urgent need for a quick and easily available biosensor for detection. Research has shown that using real-time RT-PCR assay with molecular beacons can improve the accuracy of detecting SARS-CoV-2 and distinguish between pathogenic and non-pathogenic strains of coronaviruses.