Journal
MOLECULAR BIOTECHNOLOGY
Volume 64, Issue 5, Pages 575-589Publisher
SPRINGERNATURE
DOI: 10.1007/s12033-021-00434-4
Keywords
Methicillin-resistant Staphylococcus aureus; Docosanol; Two-dimensional electrophoresis; Proteome; Antioxidants; Oxidative stress
Funding
- University Grants Commission for Mid-Career Award [F.19-225/2018(BSR), F.24-51/2014-U]
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This study found that docosanol affects the protein expression profile of MRSA by reducing the expression of bacillithiol and stress-associated proteins, effectively targeting the antioxidant pathway.
The present study was aimed to investigate the effect of docosanol on the protein expression profile of methicillin-resistant Staphylococcus aureus (MRSA). Thus, two-dimensional gel electrophoresis coupled with MALDI-TOF MS technique was utilized to identify the differentially regulated proteins in the presence of docosanol. A total of 947 protein spots were identified from the intracellular proteome of both control and docosanol treated samples among which 40 spots were differentially regulated with a fold change greater than 1.0. Prominently, the thiol-dependent antioxidant system and stress response proteins are downregulated in MRSA, which are critical for survival during oxidative stress. In particular, docosanol downregulated the expression of Tpx, AhpC, BshC, BrxA, and YceI with a fold change of 1.4 (p = 0.02), 1.4 (p = 0.01), 1.6 (p = 0.002), 4.9 (p = 0.02), and 1.4 (p = 0.02), respectively. In addition, docosanol reduced the expression of proteins involved in purine metabolic pathways, biofilm growth cycle, and virulence factor production. Altogether, these findings suggest that docosanol could efficiently target the antioxidant pathway by reducing the expression of bacillithiol and stress-associated proteins.
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