Journal
MOLECULAR BIOLOGY REPORTS
Volume 49, Issue 3, Pages 2325-2333Publisher
SPRINGER
DOI: 10.1007/s11033-021-06963-0
Keywords
RNA sequencing; Gene-related diseases; Transcriptomes; Eukaryotic organisms; Short-read; Long-read
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With the development of RNA sequencing technology, the diagnosis and identification of gene-related diseases have become more accurate. It has also provided a better understanding of rare diseases and taxonomical classifications. RNA sequencing can help detect resistant genes and improve treatment methods for higher effectiveness and fewer side effects.
With the invention of RNA sequencing over a decade ago, diagnosis and identification of the gene-related diseases entered a new phase that enabled more accurate analysis of the diseases that are difficult to approach and analyze. RNA sequencing has availed in-depth study of transcriptomes in different species and provided better understanding of rare diseases and taxonomical classifications of various eukaryotic organisms. Development of single-cell, short-read, long-read and direct RNA sequencing using both blood and biopsy specimens of the organism together with recent advancement in computational analysis programs has made the medical professional's ability in identifying the origin and cause of genetic disorders indispensable. Altogether, such advantages have evolved the treatment design since RNA sequencing can detect the resistant genes against the existing therapies and help medical professions to take a further step in improving methods of treatments towards higher effectiveness and less side effects. Therefore, it is of essence to all researchers and scientists to have deeper insight in all available methods of RNA sequencing while taking a step-in therapy design.
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