Morphological and molecular characteristics of fungal species associated with crown rot of strawberry in South Korea

Background Crown and root rot is the most important and destructive strawberry diseases in Korea as it causes substantial economic loss. In August 2020, a severe outbreak of crown and root rot on strawberries (Fragaria x ananassa Duch.) was observed in the greenhouse at Sangju, South Korea. Infected plantlets displayed browning rot within the crown and root, stunted growth, and poor rooting. Methods and results Thirty fungal isolates were obtained from the affected plantlet. Isolates were identified based on morphological characteristics and pathogenicity test as well as sequence data obtained from internal transcribed spacer, large subunit ribosomal ribonucleic acid, translation elongation factor, and RNA polymerase II-second largest subunit. Results showed that the crown and root rot of strawberry in Korea was caused by three distinct fungal species: Fusarium oxysporum f. sp. fragariae, F. solani, and Plectosphaerella cucumerina. To the best of our knowledge, F. solani, and P. cucumerina are reported for the first time as the causal agents of the crown and root rot of strawberry in South Korea. Pathogenicity tests confirmed that these isolates are pathogenic to strawberry. Conclusions Understanding the composition and biology of the pathogen population will be helpful to provide effective control strategies for the disease.

Automated summary

A severe outbreak of crown and root rot on strawberries in a greenhouse in Sangju, South Korea, was caused by three distinct fungal species: Fusarium oxysporum f. sp. fragariae, F. solani, and Plectosphaerella cucumerina, with F. solani and P. cucumerina being reported for the first time as causal agents in South Korea. Pathogenicity tests confirmed the isolates' ability to cause disease in strawberries. Understanding the pathogen population's composition and biology is essential for effective disease control strategies.