The fluorescent probe-based recombinase-aided amplification for rapid detection of Escherichia coli O157:H7

Escherichia coli O157:H7 (E. coli O157:H7) is a common foodborne morbigenous microorganism, which can spread through fecal-oral transmission. Humans can be infected by ingesting foods and water contaminated with E. coli O157:H7, which can cause various symptoms. In present study, we have successfully developed a quick and hypersensitive fluorescent probe-based Recombinase-aided amplification (RAA) method and applied in E. coli O157:H7 detection at 39 degrees C in 20 min. The sensitivity of the assay in pure E. coli O157:H7 suspension was 5.6 x 100 CFU/mL. The fluorescent probe-based RAA assay was further applied in three samples, and the limit of detection (LOD) in skimmed milk, lettuces and lake water was 5.4 x 101 CFU/mL, 7.9 x 101 CFU/mL and 5.2 x 101 CFU/mL, separately. This method showed a high sensitivity and short detection time, which has the feasible application in on-site test in real samples.

Automated summary

A quick and highly sensitive fluorescent probe-based RAA method was developed and successfully applied in detecting E.coli O157:H7, showing high sensitivity and feasibility for on-site testing in real samples.