Journal
MICROSCOPY RESEARCH AND TECHNIQUE
Volume 85, Issue 5, Pages 1949-1955Publisher
WILEY
DOI: 10.1002/jemt.24056
Keywords
histidine-doped carbon nanodots; oxidase-mimicking nanozymes; pathogenic E; coli
Categories
Funding
- MEXT [T20K05260]
Ask authors/readers for more resources
This study demonstrates that histidine-containing carbon nanodots can serve as externally tunable antibacterial agents when irradiated with visible light. The carbon nanodots with positive charge can readily adsorb on the negatively charged envelope of bacteria under slightly acid pH, thereby inhibiting their growth under visible light illumination.
Here we demonstrate the nanozyme properties of histidine-containing carbon nanodots as externally tunable antibacterial agents through irradiation with visible (VIS) light. The correlative (light and electron) microscopic analysis of treated Escherichia coli O157:H7 revealed that the positive charged carbon nanoparticles might readily adsorb at slightly acid pH on the negative charged cellular envelope of bacteria, and thus, inhibit their growth with over 80% efficiency under illumination with VIS light. The reason was that under VIS irradiation in the range 400-500 nm the adsorbed nanoparticles behaved as effective oxidase-mimicking enzymes and generated reactive oxygen species on the labeled cells. Thus, the light-activated artificial nanozyme caused serious physical damaging of bacterial envelope, which was leading to irreversible cellular inhibition. The outcomes of this study are likely to broaden the scope of designed photoactive carbon nanozymes as powerful antibacterial agents against the emergence of antibiotic and multidrug-resistant strains, as well as proposing of new strategies for infection control.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available