Journal
MICROSCOPY AND MICROANALYSIS
Volume 28, Issue 2, Pages 527-536Publisher
OXFORD UNIV PRESS
DOI: 10.1017/S1431927622000162
Keywords
atomic force microscopy; casein micelle structure; cryo-transmission electron microscopy; multivariate analysis; Raman spectroscopy
Categories
Funding
- CONACyT
- BEIFI
- Instituto Politecnico Nacional (IPN) in Mexico City
- CONACyT [239899, 268660]
- Secretaria de Investigacion y Posgrado at IPN [20180455, 20195198, 20200505, 20200506]
Ask authors/readers for more resources
This study evaluated the influence of pH changes on the morphometric parameters and conformational structure of casein micelles. It was found that the morphology and secondary structure of casein micelles strongly depend on pH. Atomic force and cryo-transmission electron microscopy were used to characterize the changes in average roughness, size, and shape of the micelles at different pH levels. Raman spectroscopy was also used to evaluate the folding and unfolding of casein molecules, which correlated well with the morphometric changes of the micelles.
This study aimed to evaluate the influence of pH changes on morphometric parameters of casein micelles and a general overview of their conformational structure through microscopy techniques, Raman spectroscopy and multivariate analysis. It was found that casein micelles morphology and protein secondary structure depend strongly upon pH. The changes of arithmetic average roughness (Ra), size, and shape of casein micelles at different pH are properly characterized by atomic force and cryo-transmission electron microscopy. Morphometric changes of casein micelles were correlated correctly with folding and unfolding of casein molecules as evaluated by Raman spectroscopy when the pH was varied. The novelty of this contribution consists in demonstrating that there is a close structure-functionality relationship between the morphometric parameters of proteins and their secondary structure. Knowledge about casein micelles can help improve their use of its diverse applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available