Journal
INDUSTRIAL CROPS AND PRODUCTS
Volume 89, Issue -, Pages 543-549Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.indcrop.2016.05.010
Keywords
Anti-inflammatory activity; Antioxidant activity; Chemical composition analysis; Lonicera japonica; Supercritical fluid extraction
Categories
Funding
- Ministry of Economic Affairs, Taiwan [SBIR 2Z1010479]
- I-Shou University, Taiwan [ISU101-INT-002]
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Lonicera japonica Thunberg (LJ) has long been used as an antipyretic, anti-inflammatory and anti infectious agent in East Asia. The subspecies L. japonica Thunb. var. sempervillosa Hayata (LJv) is a variant that mainly grows in Taiwan. This study examined the antioxidant and anti-inflammatory activities of the extracts from the flower buds of these two species. The extracts were obtained by three extraction methods: water extraction, ethanol extraction, and supercritical-CO2 fluid extraction (SFE). The antioxidant activities of dry LJ (dLJ) extracts were superior to those of LJv extracts. Water extracts possessed higher activities than that prepared by ethanol or SFE. The total polyphenols content, total flavonoids content, and the amount of chlorogenic acid and luteolin-7-O-glucoside were all higher in the water extracts compared to the other two. The SFE extracts of these two species all exhibited excellent anti-inflammatory activities. Although the water and ethanol extracts of dLJ extracts had higher anti-inflammatory activity than that of LJv extracts, the SFE extracts prepared from fresh LJv flower buds (fLJv) exhibited the highest activity among all extracts. The SFE effectively isolates the bioactive components of L. japonica and can obtain the L japonica extracts with high anti-inflammatory activity. (C) 2016 Elsevier B.V. All rights reserved.
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