Journal
MICROCHIMICA ACTA
Volume 189, Issue 1, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-021-05145-w
Keywords
Breast cancer; CA15-3; Layer-by-layer; Gold nanoparticles; Reduced graphene oxide; Electrochemical immunosensor
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Funding
- Brazilian agency: Coordination of Superior Level Staff Improvement (CAPES) [001, 88887.334689/2019-00]
- Brazilian agency: Sao Paulo Research Foundation (FAPESP) [2019/13514-9, 2018/22214-6, 2019/01777-5]
- Brazilian agency: CNPq
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In this study, a sandwich-type electrochemical immunosensor was developed for sensitive detection of CA15-3, with an ultra-low limit of detection and a wide linear concentration range. The immunosensor showed high selectivity, stability, and rapid detection of CA15-3 in different biological fluids.
A sensitive detection of carbohydrate antigen 15-3 (CA15-3) levels may allow for early diagnosis and monitoring the treatment of breast cancer, but this can only be made in routine clinical practice if low-cost immunosensors are available. In this work, we developed a sandwich-type electrochemical immunosensor capable of rapid detection of CA15-3 with an ultra-low limit of detection (LOD) of 0.08 fg mL(-1) within a wide linear concentration range from 0.1 fg mL(-1) to 1 mu g mL(-1). The immunosensor had a matrix of a layer-by-layer film of Au nanoparticles and reduced graphene oxide (Au-rGO) co-electrodeposited on screen-printed carbon electrodes (SPCE). The high sensitivity was achieved by using secondary antibodies (Ab(2)) labeled with horseradish peroxidase (HRP) in the presence of hydrogen peroxide (H2O2) as signal amplifiers, and hydroquinone (HQ) was used as an electron mediator. The immunosensor was selective for CA15-3 in human serum and artificial saliva samples, robust, and stable to permit storage at 4 degrees C for more than 30 days. With its high performance, the immunosensor may be incorporated into future point-of-care (POC) devices to determine CA15-3 in distinct biological fluids, including in blood and saliva samples.
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