4.7 Article

Lateral flow colorimetric biosensor for detection of Vibrio parahaemolyticus based on hybridization chain reaction and aptamer

Journal

MICROCHIMICA ACTA
Volume 188, Issue 11, Pages -

Publisher

SPRINGER WIEN
DOI: 10.1007/s00604-021-05031-5

Keywords

Vibrio parahaemolyticus; Aptamer; Magnetic nanoparticles; Hybridization chain reaction; Lateral flow nucleotide biosensor; Colorimetric detection

Funding

  1. Central Public-interest Scientific Institution Basal Research Fund, CAFS [2020TD41]
  2. Agriculture Research System of Shanghai, China [201405]

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A new detection assay for Vibrio parahaemolyticus was introduced, utilizing aptamer combined with magnetic nanoparticles, hybridization chain reaction, and lateral flow nucleotide biosensor. It showed high specificity and fast detection time, making it suitable for low-resource laboratories or districts.
Vibrio parahaemolyticus (V. parahaemolyticus) is the causative agent for acute hepatopancreatic necrosis disease (AHPND) of shrimp, and it is also a common seafood-borne pathogen for humans. Rapid and accurate identification of V. parahaemolyticus is helpful to diagnose the AHPND and ensure food safety. Common detection methods suffer the deficiency of time-consuming and complexed operation. Based on the increased development of aptamer and our previous study, a new detection assay of V. parahaemolyticus was introduced, in which the aptamer combined with magnetic nanoparticles (MNPs) was the recognizer, hybridization chain reaction (HCR) was the signal amplifier, and lateral flow nucleotide biosensor (LFNB) was the signal exporter. The assay possessed high specificity of distinguishing the target with other bacteria, and the calculated limit of detection was 2.6 x 10(3) cells. Furthermore, the whole process just needs 67 min, free of thermocycle instruments and signal readout instruments, which means it is suitable for low-resource laboratories or districts.

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