Detection of Listeria monocytogenes based on teicoplanin functionalized magnetic beads combined with fluorescence assay

Article Chemistry, Applied

A sensitive immunosensor based on FRET between gold nanoparticles and InP/ZnS quantum dots for arginine kinase detection

Yanbo Wang et al.

Summary: A novel immunoassay for quantitation of shrimp arginine kinase (AK) was developed using gold nanoparticles and quantum dots, showing high sensitivity and specificity.

FOOD CHEMISTRY (2021)

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Review Biochemistry & Molecular Biology

Making Sense of the Biodiversity and Virulence of Listeria monocytogenes

Olivier Disson et al.

Summary: Listeria monocytogenes is a foodborne pathogen causing listeriosis, with manifestations such as bacteremia and meningoencephalitis. Reference strains have been crucial in microbiological, immunological, and cell biology research. Integrating bacterial population genomics with environmental, epidemiological, and clinical data has revealed new factors in L. monocytogenes' virulence and adaptation.

TRENDS IN MICROBIOLOGY (2021)

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Article Biophysics

An ultrasensitive CRISPR/Cas12a based electrochemical biosensor for Listeria monocytogenes detection

Fan Li et al.

Summary: The study introduces the trans-cleavage activity of CRISPR/Cas12a into an electrochemical biosensor (ECRISPR) combined with recombinase-assisted amplification (RAA) to develop a cost-effective, specific, and ultrasensitive method for Listeria monocytogenes detection. The RAA-based E-CRISPR sensor can detect as low as 0.68 aM of genomic DNA and 26 cfu/mL of L. monocytogenes in pure cultures, demonstrating rapid and ultrasensitive detection capabilities in spiked and natural samples. This system shows no cross-reactivity with other non-target bacteria, making it a simple, highly sensitive, and accurate platform for L. monocytogenes detection.

BIOSENSORS & BIOELECTRONICS (2021)

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Article Food Science & Technology

Development of a fluorescence aptasensor for rapid and sensitive detection of Listeria monocytogenes in food

Rui Liu et al.

Summary: In this study, a simple fluorescence aptasensor was developed for rapid and sensitive detection of Listeria monocytogenes in food. The sensor showed a low limit of detection and wider detection range compared to other methods, with good selectivity and functionality in detecting L. monocytogenes in real food samples.

FOOD CONTROL (2021)

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Article Food Science & Technology

Development of a rapid Salmonella detection method via phage-conjugated magnetic bead separation coupled with real-time PCR quantification

Chenxi Huang et al.

Summary: The study developed a rapid and accurate detection method for Salmonella using the lytic Siphovirus phage LPST10, which showed a detection limit of <30 CFU/mL in different food matrices. The method involves magnetic separation and real-time PCR detection, allowing for quick and efficient detection of Salmonella in complex food samples.

LWT-FOOD SCIENCE AND TECHNOLOGY (2021)

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Article Food Science & Technology

Real-Time PCR Method Combined with a Matrix Lysis Procedure for the Quantification of Listeria monocytogenes in Meat Products

Mirian Labrador et al.

Summary: A real-time PCR method has been developed for quantifying Listeria monocytogenes on meat products through the gene hlyA, achieving high efficiency and specificity. The method, combined with matrix lysis, showed excellent correspondence with the reference method and higher precision and sensitivity at low concentrations of the pathogen, demonstrating potential for routine control in the meat industry.

FOODS (2021)

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Article Chemistry, Medicinal

A facile method for vancomycin C-terminus functionalization and derivatization through hydrazide

Yu Hu et al.

Summary: This study presents a facile strategy for functionalization and derivatization of vancomycin's C-terminus. By introducing C-terminal hydrazide on vancomycin, the researchers successfully conjugated cysteine and cysteine containing peptides onto vancomycin C-terminus, preparing two fluorescent FITC-vancomycin with good antibacterial activity. Additionally, lipophilic structures were introduced onto vancomycin C-terminus, resulting in vancomycin derivatives that were evaluated against both Gram-positive and negative bacteria strains.

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS (2021)

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Article Food Science & Technology