Label-free electrochemical immunosensor for ultrasensitive determination of cardiac troponin I based on porous fluffy-like AuPtPd trimetallic alloyed nanodendrites

Enhancement in the signals of electrochemical immunosensors via advanced nanomaterials is beneficial for developing immunoassay of cardiac troponin I (cTnI) with high sensitivity and selectivity. By virtue of K-3[Fe(CN)(6)] as a signal probe, an advanced label-free electrochemical immunosensor was fabricated for ultrasensitive detection of cTnI with trimetallic alloyed AuPtPd porous fluffy-like nanodendrites (AuPtPd FNDs), followed by assembly of the antibody (Ab). By virtue of the structural advantages, electronic effects and synergetic catalysis of the trimetals, such nanostructures showed large specific surface area to increase the loading of the cTnI Ab and amplify the electrochemical signals of the probe. The sensor displayed a wide linear range (0.01 similar to 100.0 ng mL(-1)) and a low detection limit (LOD = 3 pg mL(-1)) under the optimal conditions, which showed great potential for detecting cTnI in serum samples. Furthermore, this biosensor provides an effective detection platform for analysis of other cardiac markers in practical samples.

Automated summary

Enhancement of electrochemical immunosensors using advanced nanomaterials is beneficial for developing high-sensitivity and high-selectivity immunoassays for cardiac troponin I (cTnI). By utilizing trimetallic alloyed AuPtPd porous fluffy-like nanodendrites (AuPtPd FNDs) as the assembly point for the antibody (Ab), the sensor displayed a wide linear range (0.01 to 100.0 ng/mL) and a low detection limit (LOD = 3 pg/mL), showing great potential for detecting cTnI in serum samples and providing an effective platform for analysis of other cardiac markers in practical samples.