Journal
MICROBIOLOGY-SGM
Volume 167, Issue 12, Pages -Publisher
MICROBIOLOGY SOC
DOI: 10.1099/mic.0.001123
Keywords
sponge; natural product discovery; hexactinellid; antimicrobial; culture
Categories
Funding
- Society for Applied Microbiology
- School of Biological and Marine Sciences, University of Plymouth
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Through culture-based microbial analysis of deep-sea sponges, this study discovered novel antimicrobial candidates and demonstrated antimicrobial activity, showcasing the potential for screening novel antimicrobial compounds.
Access to deep-sea sponges brings with it the potential to discover novel antimicrobial candidates, as well as novel cold- and pressure-adapted bacteria with further potential clinical or industrial applications. In this study, we implemented a combination of different growth media, increased pressure and high-throughput techniques to optimize recovery of isolates from two deep-sea hexactinellid sponges, Pheronema carpenteri and Hertwigia sp.. in the first culture-based microbial analysis of these two sponges. Using 16S rRNA gene sequencing for isolate identification, we found a similar number of cultivable taxa from each sponge species, as well as improved recovery of morphotypes from P carpenteri at 22-25 degrees C compared to other temperatures, which allows a greater potential for screening for novel antimicrobial compounds. Bacteria recovered under conditions of increased pressure were from the phyla Proteobacteria, Actinobacteria and Firmicutes, except at 4%O-2/5 bar, when the phylum Firmicutes was not observed. Cultured isolates from both sponge species displayed antimicrobial activity against Micrococcus luteus, Staphylococcus aureus and Escherichia coli.
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