Journal
MICROBIOLOGICAL RESEARCH
Volume 254, Issue -, Pages -Publisher
ELSEVIER GMBH
DOI: 10.1016/j.micres.2021.126896
Keywords
Nitrogen fixation; Biotechnology; Agriculture; Genomics
Categories
Funding
- Fundacao Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ) [E-26/111.827/2013, E-26/102.259/2013, E-26/201.239/2014, E-26/203.309/2016, E-26/203.014/2018]
- Coordenacao de Aperfeicoa-mento de Pessoal de Nivel Superior-Brasil (CAPES) [001]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [449904/2014-8, 314263/2018-7]
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Our research group isolated a new strain, Azospirillum sp. UENF412522, from passion fruit rhizoplane, demonstrating its ability to promote the growth of passion fruit plants. This strain has the capabilities of nitrogen fixation, phosphate solubilization, and indole-acetic acid production, as well as resistance to common soil antibiotics. Genome sequencing revealed key genes responsible for its plant growth promotion properties, making it a promising candidate for bioinoculant formulations.
Given their remarkable beneficial effects on plant growth, several Azospirillum isolates currently integrate the formulations of various commercial inoculants. Our research group isolated a new strain, Azospirillum sp. UENF412522, from passion fruit rhizoplane. This isolate uses carbon sources that are partially distinct from closelyrelated Azospirillum isolates. Scanning electron microscopy analysis and population counts demonstrate the ability of Azospirillum sp. UENF-412522 to colonize the surface of passion fruit roots. In vitro assays demonstrate the ability of Azospirillum sp. UENF-412522 to fix atmospheric nitrogen, to solubilize phosphate and to produce indole-acetic acid. Passion fruit plantlets inoculated with Azospirillum sp. UENF-41255 showed increased shoot and root fresh matter by 13,8% and 88,6% respectively, as well as root dry matter by 61,4%, further highlighting its biotechnological potential for agriculture. We sequenced the genome of Azospirillum sp. UENF-412522 to investigate the genetic basis of its plant-growth promotion properties. We identified the key nif genes for nitrogen fixation, the complete PQQ operon for phosphate solubilization, the acdS gene that alleviates ethylene effects on plant growth, and the napCAB operon, which produces nitrite under anoxic conditions. We also found several genes conferring resistance to common soil antibiotics, which are critical for Azospirillum sp. UENF-412522 survival in the rhizosphere. Finally, we also assessed the Azospirillum pangenome and highlighted key genes involved in plant growth promotion. A phylogenetic reconstruction of the genus was also conducted. Our results support Azospirillum sp. UENF-412522 as a good candidate for bioinoculant formulations focused on plant growth promotion in sustainable systems.
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