Journal
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 130, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.msec.2021.112460
Keywords
Photo-crosslinkable hydrogels; Methacrylamide-modified collagen; Methacrylamide-modified gelatin; Vascular tissue engineering
Categories
Funding
- Vanier Canada Graduate Scholarship
- Research Foundation Flanders (FWO)
- Natural Science and Engineering Research Council of Canada
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This study developed COL-MA and GEL-MA, collagen-based hydrogel precursors functionalized for photo-crosslinking, providing a new approach for vascular tissue engineering applications. The derivatives showed high crosslinking efficiency, tunable mechanical properties, and superior cell interactions compared to GEL-MA.
The present work targets the development of collagen-based hydrogel precursors, functionalized with photo-crosslinkable methacrylamide moieties (COL-MA), for vascular tissue engineering (vTE) applications. The developed materials were physico-chemically characterized in terms of crosslinking kinetics, degree of modifi-cation/conversion, swelling behavior, mechanical properties and in vitro cytocompatibility. The collagen de-rivatives were benchmarked to methacrylamide-modified gelatin (GEL-MA), due to its proven track record in the field of tissue engineering. To the best of our knowledge, this is the first paper in its kind comparing these two methacrylated biopolymers for vTE applications. For both gelatin and collagen, two derivatives with varying degrees of substitutions (DS) were developed by altering the added amount of methacrylic anhydride (MeAnH). This led to photo-crosslinkable derivatives with a DS of 74 and 96% for collagen, and a DS of 73 and 99% for gelatin. The developed derivatives showed high gel fractions (i.e. 74% and 84%, for the gelatin derivatives; 87 and 83%, for the collagen derivatives) and an excellent crosslinking efficiency. Furthermore, the results indicated that the functionalization of collagen led to hydrogels with tunable mechanical properties (i.e. storage moduli of [4.8-9.4 kPa] for the developed COL-MAs versus [3.9-8.4 kPa] for the developed GEL-MAs) along with superior cell-biomaterial interactions when compared to GEL-MA. Moreover, the developed photo-crosslinkable collagens showed superior mechanical properties compared to extracted native collagen. Therefore, the developed photo-crosslinkable collagens demonstrate great potential as biomaterials for vTE applications.
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