Journal
BIOMATERIALS ADVANCES
Volume 133, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.msec.2021.112608
Keywords
Quaternized carbon quantum dots; Enhance antibacterial activities; bacteria resistance; Promote wound healing
Categories
Funding
- National Natural Science Foundation of China [82072379]
- Natural Science Foundation of Fujian Province [2019J01308]
- Joint Funds for the Innovation of Science and Technology, Fujian Province [2017Y9124]
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The synthesis of quaternized carbon quantum dots (Q-CQDs) derived from curcumin and GTA enhances the bioactivity and broad-spectrum antibacterial effects of curcumin. Q-CQDs show excellent antibacterial activity without bacterial resistance. The antibacterial mechanism involves the adherence of Q-CQDs to bacterial cell membranes, ROS generation, and the efflux of DNA and RNA.
With the increasing incidence of multidrug-resistant antibacterial infections worldwide, developing new antibiotics to fight bacterial infections is urgent. The natural product curcumin has favorable antioxidant and anti-inflammatory effects, but poor water solubility greatly limits its bioavailability, bioactivity and clinical application. Herein, to improve the bioactivity and enhance broad-spectrum antibacterial of curcumin, we synthesized quaternized carbon quantum dots (Q-CQDs) derived from the natural curcumin and 2,3-epoxypropyltrimethylammonium chloride (GTA) with highly solubility and stability by double-thermal method. It is proposed that the surfaces of Q-CQDs would still remain the active groups of curcumin and quaternary ammonium to boost the antibacterial activity. Experimental results reveal that the Q-CQDs possess excellent broad-spectrum antibacterial activity and the activity is significantly higher than that of natural curcumin. Investigation of the antibacterial mechanism of Q-CQDs showed that Q-CQDs functionalized with -N+(CH3)(3) had strong adherence behavior on the bacterial cell membrane. Like a Trojan Horse, the bacterial cells lost their integrity, and the entry of Q-CQDs caused ROS generation and the efflux of cytoplasmic DNA and RNA, leading to the death of bacteria. The bacterial resistance of Q-CQDs was not observed, and Q-CQDs did not cause hemolysis and cytotoxicity. In vivo, the S. aureus-infected wounds, E. coli-infected wounds and mixed bacteria infected wounds healing tests with mice model indicate that Q-CQDs inhibited the bacterial population at the wound site, reduced inflammation and promoted wound healing. These results suggested that the Q-CQDs are a potential antibacterial candidate for clinical infected-wound healing applications and even bacteria resistant infections.
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