Multifunctional 3D printed porous GelMA/xanthan gum based dressing with biofilm control and wound healing activity

Bacterial infections are the major challenges of wound treatment in current clinical applications. In this study, Three-dimensional (3D) antibacterial wound dressing has been fabricated via introducing N-halamine/TiO2 to gelatin methacrylate and xanthan gum. The prepared 3D printed dressings showed ideal swelling ratio and excellent water uptake efficiency. TiO2 nanoparticles were introduced by in-situ to improve the ultraviolet stability of N-halamines. The 3D printed GX(2)-TiO2-PSPH-Cl prepared dressings containing titanium dioxide retained 0.19% active chlorine after ultraviolet irradiation for 20 min, which was much higher than that of N-halamine dressings without the addition of TiO2. The 3D printed dressings showed good antibacterial activity, and 100% of Escherichia coli O157:H7 and Staphylococcus aureus were inactivated after 60 min of contact. Furthermore, the biofilm test indicated that the 3D antibacterial dressings were able to inhibit the formation of bacterial biofilm. The 3D printed dressings possess outstanding biocompatibility. Moreover, in vivo data demonstrated that the 3D printed dressings could significantly accelerate wound healing in a mouse model, indicating that the developed 3D printed dressings are ideal candidates for wound treatment.

Automated summary

The 3D printed antibacterial wound dressing with N-halamine/TiO2 showed excellent water uptake efficiency and antibacterial activity against Escherichia coli and Staphylococcus aureus, while inhibiting bacterial biofilm formation. In vivo data demonstrated accelerated wound healing in a mouse model, indicating the potential of these dressings for wound treatment.