Journal
LIFE SCIENCES
Volume 290, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.lfs.2021.120266
Keywords
Berberine; Gastric cancer; IL-6; JAK2; STAT3; RNA-sequencing
Funding
- National Key Research and Development Program of China [2017YFC1702600, 2017YFC1702605]
- County-University cooperation Innovation Funds of Southwest University [SZ201901]
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Berberine was found to inhibit proliferation, induce apoptosis and cell cycle arrest of gastric cancer cells, as well as suppress migration and invasion. In addition, it was shown to modulate signaling pathways related to IL-6/JAK2/STAT3, providing a theoretical basis for its use in gastric cancer prevention.
Aim: Gastric cancer is a prevalent malignant tumor that seriously affects human health. Berberine (BBR), an alkaloid from Chinese herbal medicines, inhibits the proliferation of various cancers. We evaluated the effects and related mechanisms of BBR on gastric cancer.Main methods: The MTT assay, flow cytometry, scratch assays, transwell experiments and xenograft nude mice models were used to investigate the antineoplastic effects of BBR. RNA-Seq, qRT-PCR, WB and ELISA were used to investigate the underlying mechanisms of BBR on gastric cancer metastasis. Key findings: BBR treatment inhibited the proliferation of MKN-45 and HGC-27 cells, induced their apoptosis, G0/ G1 cell arrest, and suppressed the migration as well as invasion of GC cells in vitro. Moreover, BBR inhibited in vivo tumor growth in MKN-45 xenograft mice. RNA-seq showed that interactions between cytokines and their receptors was one of the greatest enrichment modulated pathways and IL-6 was a key target. IL-6 knockdown significantly inhibited the activities of MKN-45 cells. Mechanistically, these findings imply that BBR inhibits GC cell proliferation by modulating the signaling pathways related to IL-6/JAK2/STAT3.Significance: This study provides a theoretical basis for the use of BBR in gastric cancer prevention.
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