Compost as an untapped niche for thermotolerant yeasts capable of high-temperature ethanol production

Efficient bioethanol production from lignocellulosic biomass requires thermotolerant yeasts capable of utilizing multiple sugars, tolerating inhibitors and fermenting at high temperatures. In this study, 98 thermotolerant yeasts were isolated from nine compost samples. We selected 37 yeasts that belonged to 11 species; 31 grew at 45 degrees C; 6 strains grew at 47 degrees C, while 9 yeasts could utilize multiple sugars. Many yeast isolates showed high ethanol production in the range of 12-24 g l(-1), with fermentation efficiencies of 47-94% at 40 degrees C using 5% glucose. Kluyveromyces marxianus CSV3.1 and CSC4.1 (47 degrees C), Pichia kudriavzevii CSUA9.3 (45 degrees C) produced 21, 22 and 23 g l(-1) of ethanol with efficiencies of 83, 87 and 90%, respectively, using 5% glucose. Among these yeasts, K. marxianus CSC4.1 and P. kudriavzevii CSUA9.3 exhibited high tolerance against furfural, 5-HMF, acetic acid and ethanol. These two strains produced high amounts of ethanol from alkali-treated RS, with 84 and 87% efficiency via separate hydrolysis and fermentation; 76 and 74% via simultaneous saccharification and fermentation at 47 and 45 degrees C, respectively. Therefore, this study demonstrates compost as a potential anthropogenic niche for multiple sugar-utilizing, inhibitor-tolerant ethanologenic yeasts suitable for high-temperature ethanol production via SHF of rice straw.

Automated summary

This study isolated thermotolerant yeasts capable of utilizing multiple sugars and tolerating inhibitors from compost samples, with strains such as Kluyveromyces marxianus and Pichia kudriavzevii showing high ethanol production efficiency. These yeasts demonstrated potential for high-temperature ethanol production from lignocellulosic biomass.