Journal
LETTERS IN APPLIED MICROBIOLOGY
Volume 74, Issue 5, Pages 632-639Publisher
OXFORD UNIV PRESS
DOI: 10.1111/lam.13649
Keywords
CEACAM-1; exopolysaccharides; influenza virus; Lactobacillus delbrueckii ssp; bulgaricus OLL1073R-1; Staphylococcus aureus
Categories
Funding
- JSPS KAKENHI [20K08846, 21K08287]
- Showa University
- Grants-in-Aid for Scientific Research [20K08846, 21K08287] Funding Source: KAKEN
Ask authors/readers for more resources
This study found that exopolysaccharides (EPS) produced by Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 can inhibit influenza virus infection and secondary bacterial infection. The treatment with EPS before viral infection can decrease the expression of CEACAM-1, leading to reduced virus titre and bacterial adherence.
The present study assessed the inhibitory action of exopolysaccharides (EPS) produced by Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 against influenza virus infection followed by secondary bacterial infection. We found that the presence of 200 or 400 mu g ml(-1) of EPS significantly protected against influenza virus infection in a dose-dependent manner when A549 cells were treated with EPS before infection but not after it. The expression of carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM-1), an adhesion molecule for bacteria adherence, on A549 cells was significantly enhanced during influenza virus infection compared with viral-non-infected A549 cells. However, this upregulated CEACAM-1 expression was significantly decreased by EPS treatment before viral infection in association with the reduction in the virus titre in A549 cells. In a bacterial adhesion assay using Staphylococcus aureus, the bacterial adherence to viral-infected A549 cells was significantly greater than that to viral-non-infected A549 cells, and the increased bacterial adherence induced by influenza virus infection tended to be decreased by EPS treatment before the infection. Our findings show that EPS treatment before viral infection can inhibit influenza virus infection and alleviate secondary bacterial infection through decreased CEACAM-1 expression.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available