Journal
LANGMUIR
Volume 37, Issue 40, Pages 11900-11908Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.langmuir.1c02030
Keywords
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Funding
- FONCYT [PICT 2015-0662]
- Sao Paulo Research Foundation (FAPESP)
- CONICET
- BEPE [2018/14215-2]
- SECyTUNC
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [18/14215-2] Funding Source: FAPESP
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Hopanoids are proposed as sterol surrogates in some bacteria, and their effects on lipid membranes were studied, particularly focusing on the location and effects of hopene in the membrane. The presence of hopene significantly affected the stiffness, shear viscosity, and bending dynamics of the membranes in comparison with pure phospholipid membranes.
Hopanoids are proposed as sterol surrogates in some bacteria, and it has been proved that some hopanoids are able to induce a liquid-order phase state in lipid membranes. The members of this group of molecules have diverse structures, and not all of them have been studied in detail yet. Here, we study membranes with the hopanoid hopene (hop-22 (29)-ene or diploptene), which is the product of the cycling of squalene by squalene-hopene cyclase, and thus is present in the first step of hopanoid biosynthesis. Hopene is particularly interesting because it lacks a polar head group, which opens the question of how does this molecule accommodate in a lipid membrane, and what are the effects promoted by its presence. In order to get an insight into this, we prepared monolayers and bilayers of a phospholipid with hopene and studied their properties in comparison with pure phospholipid membranes, and with the sterol cholesterol or the hopanoid diplopterol. Film stiffness, shear viscosity, and bending dynamics were very affected by the presence of hopene, while zeta-potential, generalized polarization of Laurdan, and conductivity were affected moderately by this molecule. The results suggest that at very low percentages, hopene locates parallel to the phospholipid molecules, while the excess of the hopene molecules stays between leaflets, as previously proposed using molecular dynamics simulations.
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