4.6 Article

A Simple Method for Continuous Synthesis of Bicelles in Microfluidic Systems

Journal

LANGMUIR
Volume 37, Issue 42, Pages 12255-12262

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/acs.langmuir.1c02024

Keywords

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Funding

  1. Cooperative Research Program for Agriculture Science and Technology Development, Rural Development Administration, Republic of Korea [PJ01574703]
  2. Basic Research Program - Korea Institute of Machinery and Materials [NK226E]
  3. National Research Foundation of Korea [NRF-2020K2A9A2A08000174, 2020R1I1A1A01075234]
  4. Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through Agro and Livestock Products Safety.Flow Management Technology Development Program - Ministry of Agriculture, Food and Rural Affairs (MAFRA) [118105-3]
  5. National Research Council of Science & Technology (NST), Republic of Korea [NK226E] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
  6. National Research Foundation of Korea [2020R1I1A1A01075234] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)

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A novel method for DMPC/DHPC bicelle synthesis based on a microfluidic device without heating and freezing processes is proposed, enabling the continuous manufacturing of bicelles. Experimental and analytical studies reveal critical lipid concentration and mixing time in the microfluidic system, as well as a linear relation between the actual composition of bicelle and initial lipid ratio. This method allows for controlled size of bicelles.
Bicelle has great potential for drug delivery systems due to its small size and biocompatibility. The conventional method of bicelle preparation contains a long process and harsh conditions, which limit its feasibility and damage the biological substances. For these reasons, a continuous manufacturing method in mild conditions has been demanded. Here, we propose a novel method for DMPC/DHPC bicelle synthesis based on a microfluidic device without heating and freezing processes. Bicelles were successfully prepared using this continuous method, which was identified by the physicochemical properties and morphologies of the synthesized assemblies. Experimental and analytical studies confirm that there is critical lipid concentration and critical mixing time for bicelle synthesis in this microfluidic system. Furthermore, a linear relation between the actual composition of bicelle and initial lipid ratio is deduced, and this enables the size of bicelles to be controlled.

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