Journal
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
Volume 22, Issue 11, Pages 941-953Publisher
ZHEJIANG UNIV PRESS
DOI: 10.1631/jzus.B2000818
Keywords
Ganoderma lucidum; Triterpenoid; Heme oxygenase-1 (HO-1); carbon monoxide (CO) signaling; Transcriptome sequencing
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Funding
- Applied Basic Research Project of Shanxi Province [201901D211402]
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This study demonstrated that Ganoderic triterpenoids (GTs) accumulation in Ganoderma lucidum mycelia can be enhanced by heme oxygenase-1 (HO-1)/carbon monoxide (CO) signaling. The findings suggest that HO-1/CO signaling may play a direct regulatory role in promoting GT accumulation by modulating gene expression in the mevalonate pathway and reactive oxygen species (ROS) and Ca2+ pathways. This research contributes to the understanding of GT metabolic networks and signaling pathways for potential large-scale production.
Ganoderic triterpenoids (GTs) are the primary bioactive constituents of the Basidiomycotina fungus, Ganoderma lucidum. These compounds exhibit antitumor, anti-hyperlipidemic, and immune-modulatory pharmacological activities. This study focused on GT accumulation in mycelia of G. lucidum mediated by the heme oxygenase-1 (HO-1)/carbon monoxide (CO) signaling. Compared with the control, hemin (10 mu mol/L) induced an increase of 60.1% in GT content and 57.1% in HO-1 activity. Moreover, carbon monoxide-releasing molecule-2 (CORM-2), CO donor, increased GT content by 56.0% and HO-1 activity by 18.1%. Zn protoporphyrin IX (ZnPPIX), a specific HO-1 inhibitor, significantly reduced GT content by 26.0% and HO-1 activity by 15.8%, while hemin supplementation reversed these effects. Transcriptome sequencing showed that HO-1/CO could function directly as a regulator involved in promoting GT accumulation by regulating gene expression in the mevalonate pathway, and modulating the reactive oxygen species (ROS) and Ca2+ pathways. The results of this study may help enhance large-scale GT production and support further exploration of GT metabolic networks and relevant signaling cross-talk.
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