Journal
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES
Volume 85, Issue 9, Pages 353-363Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15287394.2021.2009947
Keywords
Plinia cauliflora; pedunculagin; Ames test; CometChip; DNA repair
Funding
- National Institutes of Health [NIH R44 ES024698-01]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
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Pedunculagin (PD), an ellagitannin found in various plants, has shown pharmaceutical properties such as antitumor and antioxidant effects. This study investigated the cytotoxic, genotoxic, and antigenotoxic activities of PD using both in silico and in vitro assays. Results revealed that while PD exhibited genotoxic and cytotoxic effects in human lymphocytes, it also showed antimutagenic properties in bacteria.
Pedunculagin (PD), an ellagitannin found in different plant species, possesses several pharmaceutical properties, including antitumor, antioxidant, gastroprotective, hepatoprotective, and anti-inflammatory properties. However, the effects of PD alone on DNA remain to be determined. The aim of this study was to investigate the potential cytotoxic, genotoxic, and antigenotoxic activities of PD isolated from Plinia cauliflora seeds using in silico and in vitro assays. To elucidate the biological activities of PD, in silico tools indicative of antioxidant, antineoplastic, and chemopreventive activities of PD were used. Subsequently, the mutagenic/antimutagenic effects of PD were later assessed using bacteria with the Ames test, and the cytotoxic, genotoxic, and antigenotoxic effects utilizing human lymphocytes as evidenced by trypan blue exclusion test and CometChip assay. In silico analysis indicated potential antioxidant, chemopreventive, free radical scavenger, and cytostatic activities of PD. In the Ames test, PD was found to be not mutagenic; however, this plant component protected DNA against damage-mediated by mutagens 4-nitroquinoline-1-oxide and sodium azide. Regarding human lymphocytes, PD alone was cytotoxic and genotoxic; however, it also reduced DNA damage induced by doxorubicin at co- and post-treatment. In conclusion, PD showed genotoxic, antigenotoxic and cytotoxic effects in human lymphocytes and antimutagenic effects in bacteria.
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