4.6 Article

Maintaining flippase activity in procoagulant platelets is a novel approach to reducing thrombin generation

Journal

JOURNAL OF THROMBOSIS AND HAEMOSTASIS
Volume 20, Issue 4, Pages 989-995

Publisher

WILEY
DOI: 10.1111/jth.15641

Keywords

blood platelets; pharmacology; phosphatidylserine; phospholipid transfer proteins; thrombosis

Funding

  1. British Heart Foundation [PG/20/12/34982]
  2. BHF 4-year PhD studentship [FS/15/62/32032]

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The study revealed that R5421 can reduce platelet PS exposure and inhibit thrombin generation by maintaining flippase activity in procoagulant platelets, without directly inhibiting scramblase activity.
Background During thrombosis, procoagulant platelets expose phosphatidylserine (PS), which enhances local thrombin generation. Reducing platelet PS exposure could be a novel anti-thrombotic approach. PS is confined to the inner leaflet of the plasma membrane in unstimulated platelets by ATP-dependent flippase activity. Ca2+ ionophores trigger all platelets to expose a high level of PS by activating a scramblase protein and inactivating the flippase. Although R5421 was previously shown to reduce Ca2+ ionophore-induced PS exposure, its mechanism of action is unknown. Objectives To determine the mechanism by which R5421 reduces platelet PS exposure. Methods Washed human platelets were stimulated with the Ca2+ ionophore, A23187, to induce procoagulant platelet formation while bypassing proximal receptor signalling. Platelets PS exposure was detected using annexin V or lactadherin in flow cytometry. NBD (7-nitro-2-1,3-benzoxadiazol-4-yl)-PS was used to assess scramblase and flippase activity. Thrombin generation was monitored using a fluorogenic substrate. Results and conclusions R5421 reduced the extent of A23187-stimulated platelet PS exposure, as demonstrated with annexin V or lactadherin binding. R5421 also maintained flippase activity in procoagulant platelets. Although R5421 appeared to inhibit scramblase activity in procoagulant platelets, it did not once the flippase had been inhibited, demonstrating that scramblase activity is not directly inhibited. Furthermore, R5421 inhibited the contribution of A23187-stimulated platelets to thrombin generation. Together these data demonstrate that R5421 reduces the extent of PS exposure in procoagulant platelets by maintaining flippase activity. Maintaining flippase activity in procoagulant platelets is a novel and effective approach to reducing thrombin generation.

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