4.5 Article

Histology, physiology, and glucose and lipid metabolism of Lateolabrax maculatus under low temperature stress

Journal

JOURNAL OF THERMAL BIOLOGY
Volume 104, Issue -, Pages -

Publisher

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jtherbio.2021.103161

Keywords

Lateolabrax maculatus; Cold stress; Fatty acid content; Histological; Physiological

Funding

  1. Blue granary scientific and technological innovation of China [2019YFD0900405]
  2. National Natural Science Foundation of China [31970496]
  3. Guangdong South China Sea Key Laboratory of Aquaculture for Aquatic Economic Animals and Guangdong Ocean University [KFKT2019Y08]
  4. Program of Natural Science of Heilongjiang Province of R. P. China [LH2019C040, C2018038, ZD2018008]
  5. Harbin Normal University Graduate Innovation Project [HSDSSCX2020-14]

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This study evaluated the physiological performance and gene expression of domesticated spotted sea bass exposed to different temperatures. The results showed that the gills of the sea bass experienced pathological changes during cold stress, and there were changes in liver enzymatic activity and gene transcription levels. Lipids played a significant role in prolonged cold stress.
Spotted sea bass (Lateolabrax maculatus) is a popular and important commercial fish throughout the world, but it is unknown whether introducing domesticated fish to locations that experience cold weather might alter physiological performance. In this study, we evaluated the behavior, fatty acid content, histological analysis of liver and gills, liver enzymatic activity in carbohydrate and lipid metabolism, and gene expression in liver related to carbohydrate and lipid metabolism of spotted sea bass acclimatized at 22 degrees C (control), 16 degrees C, 10 degrees C, 8 degrees C, and 4 degrees C for 24 h, and 8 degrees C for 4 days. When L. maculatus was exposed to acute cold stress for 24 h, the gill showed curling, lamellar disorganization, lamellar epithelium hyperplasia, and formed aneurysms inside of the secondary lamellae. Long term stress over four days resulted in severe lamellar epithelium hyperplasia and curling. Continued extreme cold exposure (4 degrees C) in L. maculatus caused liver HK, PK levels and LDH activities to achieve a peak value at 0 h, and decreased over time. These indicated that glucose metabolism might play critical roles in the initial time of stress. Results of carbohydrate and lipid metabolism showed that lipids appear to play roles in prolonged cold stress. The constitutive transcriptional levels of six genes related to glucose (G6Pase) and lipid metabolism (PPAR-alpha, PPAR-gamma) and mTOR signal pathway (eif4ebp1, eif4ebp2, mlst8) genes increased significantly in most groups during cold stress.

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