4.5 Article

A comprehensive metabolomic and lipidomic analysis reveals the effect of temperature on flounder (Paralichthys olivaceus)

Journal

JOURNAL OF THERMAL BIOLOGY
Volume 104, Issue -, Pages -

Publisher

PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jtherbio.2022.103203

Keywords

Flounder; Temperature; Metabolomic; Lipidomic; Biomarker; Metabolic pathway

Funding

  1. National Natural Science Foundation of China [31872590]
  2. Fundamental Research Funds for the Central Universities [201912008, 201964019]
  3. Shandong Provincial Natural Science Foundation, China [ZR2019BCE025, ZR2019MC029]

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This study investigated the changes in endogenous compounds of flounder at different temperatures using metabolomic and lipidomic analysis, and identified potential serum biomarkers and metabolic pathways.
Temperature is a considerable stressor in aquatic environments. The objective of this study was to investigate the changes in endogenous compounds of flounder (Paralichthys olivaceus) at different temperatures based on a holistic metabolomic and lipidomic analysis. Accordingly, this study involved reversed-phase chromatography and hydrophilic interaction chromatography coupled with a mass spectrometry detection platform, and using a new database, and multivariate statistical analysis. In total, 954 unique masses were obtained, including 495 metabolites and 459 lipids, and 53 qualified differential metabolites were filtered. Specifically, among the highlighted biomarkers, 2 '-Hydroxy-5 '-methylacetophenone and FFA (17:1) were the metabolites and lipids with the largest Log2FC, respectively. In addition, 18-Hydroxycorticosterone showed the greatest downregulation. Hypoxia-inducible factor 1 signaling pathway, biosynthesis of amino acids, glycerophospholipid metabolism, and choline metabolism in cancer were enriched and noteworthy metabolic pathways, which were closely related. Overall, this study provides potential serum biomarkers and metabolic pathways for flounder as well as a reference for further studies on the physiological functions of flounder under different stressors.

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