Journal
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
Volume 34, Issue 4, Pages 533-535Publisher
ELSEVIER
DOI: 10.4103/0255-0857.195360
Keywords
Automated extraction; Candida spp.; DNA extraction; manual extraction; real-time polymerase chain reaction
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Funding
- Department of Biotechnology, Government of India [BT/PR4884/MED/29/394/2012]
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The prognosis of infected individuals with candidemia depends on rapid and precise diagnosis which enables optimising treatment. Three fungal DNA extraction protocols have been compared in this study for medically important Candida species. The quality and quantity of the DNA extracted by physical, chemical and automated protocols was compared using NanoDrop ND-2000 spectrophotometer. It was found that the yield and purity (260/230) ratio of extracted DNA was significantly high in the physical treatment-based protocol as compared to chemical based or automated protocol. Extracted DNA-based real time-polymerase chain reaction showed an analytical sensitivity of 10(3) cfu/mL. The result of this study suggests physical treatment is the most successful extraction technique compared to other two protocols.
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