4.6 Article

Primary alterations during the development of hidradenitis suppurativa

Publisher

WILEY
DOI: 10.1111/jdv.17779

Keywords

-

Categories

Funding

  1. Hungarian Research Grants [NKFIH K-128250, NKFIH PD-131689]
  2. European Union
  3. European Social Fund
  4. Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences
  5. New National Excellence Program of the Ministry for Innovation and Technology [uNKP-20-5]
  6. [EFOP-3.6.1-16-2016-00022]

Ask authors/readers for more resources

The study systematically investigated inflammatory molecules involved in three stages of HS pathogenesis, revealing that epidermal changes were already detectable in non-lesional HS skin, while inflammatory reactions were more prominent in lesional HS skin.
Background Hidradenitis suppurativa (HS) is a chronic, inflammatory disease of the apocrine gland-rich (AGR) skin region. The initial steps of disease development are not fully understood, despite intense investigations into immune alterations in lesional HS skin. Objectives We aimed to systematically investigate the inflammatory molecules involved in three stages of HS pathogenesis, including healthy AGR, non-lesional HS and lesional HS skin, with the parallel application of multiple mRNA and protein-based methods. Methods Immune cell counts (T cells, dendritic cells, macrophages), Th1/Th17-related molecules (IL-12B, TBX21, IFNG, TNFA, IL-17, IL10, IL-23A, TGFB1, RORC, CCL20), keratinocyte-related sensors (TLR2,4), mediators (S100A7, S100A8, S100A9, DEFB4B, LCN2, CAMP, CCL2) and pro-inflammatory molecules (IL1B, IL6, TNFA, IL-23A) were investigated in the three groups by RNASeq, RT-qPCR, immunohistochemistry and immunofluorescence. Results Epidermal changes were already detectable in non-lesional HS skin; the epidermal occurrence of antimicrobial peptides (AMPs), IL-1 beta, TNF-alpha and IL-23 was highly upregulated compared with healthy AGR skin. In lesional HS epidermis, TNF-alpha and IL-1 beta expression remained at high levels while AMPs and IL-23 increased even more compared with non-lesional skin. In the dermis of non-lesional HS skin, signs of inflammation were barely detectable (vs. AGR), while in the lesional dermis, the number of inflammatory cells and Th1/Th17-related mediators were significantly elevated. Conclusions Our findings that non-lesional HS epidermal keratinocytes produce not only AMPs and IL-1 beta but also high levels of TNF-alpha and IL-23 confirm the driver role of keratinocytes in HS pathogenesis and highlight the possible role of keratinocytes in the transformation of non-inflammatory Th17 cells (of healthy AGR skin) into inflammatory cells (of HS) via the production of these mediators. The fact that epidermal TNF-alpha and IL-23 appear also in non-lesional HS seems to prove these cytokines as excellent therapeutic targets.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available