Journal
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
Volume 33, Issue 3, Pages 592-597Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jasms.1c00254
Keywords
MALDI; MSI; mass spectrometry imaging; high resolution; ultrathin cryosections
Funding
- Australian Research Council (ARC)
- Malacological Society of Australasia
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In this paper, we investigate the presence of a toxin in a nudibranch and demonstrate improved resolution and sensitivity for subcellular molecular imaging. The findings suggest the potential for subcellular 3D volume reconstruction.
In this paper, we investigate the presence of latrunculin A in the outer rim of a nudibranch Chromodoris kuiteri and show that by combining ultrathin cryosection methods with MALDI MSI we can achieve improved lateral (x and y) resolution and very high resolution in the z dimension by virtue of the ultrathin 200 nm thin cryosections. We also demonstrate that a post ionization laser increases sensitivity. Recent advances in MALDI source design have improved the lateral resolution (x and y) and sensitivity during MSI. Taken together, very high z resolution, from ultrathin sections, and improved lateral (x and y) resolution will allow for subcellular molecular imaging with the potential for subcellular 3D volume reconstruction.
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