Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 144, Issue 4, Pages 1498-1502Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jacs.1c09579
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Funding
- National Institutes of Health [R01 CA227699-01, R01 AI159454]
- National Science Foundation [NSF RAPID 20-27778, CBET 20-29215]
- Zhejiang University ZJU-UIUC Joint Research Center [DREMES202001]
- Mikashi award of the Carl R. Woese Institute for Genomic Biology
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This study introduces a label-free biosensing method for quickly detecting and quantifying intact viruses in human saliva with high selectivity and sensitivity.
Several applications in health diagnostics, food, safety, and environmental monitoring require rapid, simple, selective, and quantitatively accurate viral load monitoring. Here, we introduce the first label-free biosensing method that rapidly detects and quantifies intact virus in human saliva with single-virion resolution. Using pseudotype SARS-CoV-2 as a representative target, we immobilize aptamers with the ability to differentiate active from inactive virions on a photonic crystal, where the virions are captured through affinity with the spike protein displayed on the outer surface. Once captured, the intrinsic scattering of the virions is amplified and detected through interferometric imaging. Our approach analyzes the motion trajectory of each captured virion, enabling highly selective recognition against nontarget virions, while providing a limit of detection of 1 x 10(3) copies/mL at room temperature. The approach offers an alternative to enzymatic amplification assays for point-of-collection diagnostics.
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