Journal
JOURNAL OF SEPARATION SCIENCE
Volume 45, Issue 4, Pages 919-928Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.202100751
Keywords
biological sample; macroporous monolith; polycyclic aromatic hydrocarbon; solid-phase microextraction
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Funding
- National Natural Science Foundation of China [21505115]
- Top Scientific and Technological Talents in Universities of Guizhou Province [KY2018078]
- Key Laboratory for Analytical Science of Food and Environment Pollution of Qian Xi Nan [2021-2-31]
- Science and Technology Planning Project of the Science and Technology Bureau of Qian Xi Nan Prefecture [2019-2-56]
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A novel method for the analysis of trace hydroxyl polycyclic aromatic hydrocarbons in biological samples was developed, which successfully detected several hydroxyl polycyclic aromatic hydrocarbons and achieved satisfactory recoveries in urine and whole blood samples. The use of a macroporous monolith proved to be an efficient approach for enriching trace hydroxyl polycyclic aromatic hydrocarbons in biological samples.
Hydroxyl polycyclic aromatic hydrocarbons are considered active mutagenic and carcinogenic substances and are found in extremely low levels (ng/g) in biological samples. As a result, their determination in urine and blood samples is challenging, and a sensitive and effective method for the analysis of trace hydroxyl polycyclic aromatic hydrocarbons in complex biological matrices is required. In this work, a novel macroporous in-tube solid-phase microextraction monolith was prepared via a thiol-yne click reaction, and a highly efficient analytical method based on in-tube solid-phase microextraction coupled with UHPLC-MS/MS was developed to determine hydroxyl polycyclic aromatic hydrocarbons with low detection limits of 0.137-11.0 ng/L in complex biological samples. Four hydroxyl polycyclic aromatic hydrocarbons, namely, 2-hydroxyanthraquinone, 1-hydroxypyrene, 1,8-dihydroxyanthraquinone, and 6-hydroxychrysene, were determined in the urine samples of smokers, non-smokers, and whole blood samples of mice. Satisfactory recoveries were achieved in the range of 83.1-113% with relative standard deviations of 3.2-9.7%. It was found that implementation of the macroporous monolith gave a highly efficient approach for enriching trace hydroxyl polycyclic aromatic hydrocarbons in biological samples.
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