Chiral separation using cyclodextrins as mobile phase additives in open-tubular liquid chromatography with a pseudophase coating

The chiral separation of various analytes (dichlorprop, mecoprop, ibuprofen, and ketoprofen) was demonstrated with different cyclodextrins as mobile phase additives in open-tubular liquid chromatography using a stationary pseudophase semipermanent coating. The stable coating was prepared by a successive multiple ionic layer approach using poly(diallyldimethylammonium chloride), polystyrene sulfonate, and didodecyldimethyl ammonium bromide. Increasing concentrations (0-0.2 mM) of various native and derivatized cyclodextrins in 25 mM sodium tetraborate (pH 9.2) were investigated. Chiral separation was achieved for the four test analytes using 0.05-0.1 mM beta-cyclodextrin (resolution between 1.11 and 1.34), gamma-cyclodextrin (resolution between 0.78 and 1.27), carboxymethyl-beta-cyclodextrin (resolution between 1.64 and 2.59), and 2-hydroxypropyl-beta-cyclodextrin (resolution between 0.71 and 1.76) with the highest resolutions obtained with 0.1 mM carboxymethyl-beta-cyclodextrin. %RSD values were <10%. This is the first demonstration of chiral open-tubular liquid chromatography using achiral chromatographic coatings and cyclodextrins as mobile phase additives.

Automated summary

This study demonstrates the successful chiral separation of various analytes using different cyclodextrins as mobile phase additives in open-tubular liquid chromatography with a stable coating. The method achieved high resolutions and low %RSD values.