Registration of TifGP-3 and TifGP-4 peanut germplasm lines

Late leaf spot (LLS) disease caused by Nothopassalora personata (Berk. & M.A. Curtis) U. Braun, C. Nakash, Videira & Crous is a foliar disease that plagues peanut (Arachis hypogaea L.) production worldwide. One effective solution to control this disease would be the development of resistant cultivars. IAC 322 is a breeding line resistant to LLS due to alien introgressions from A. cardenasii Krapov. & W.C. Gregory, a wild diploid relative of peanut. We crossed 'TifNV-High O/L' with IAC 322 to develop a segregating breeding population. Two lines with very high levels of resistance to LLS were selected and genotyped. Line S07 (TifGP-3, Reg. no. GP-244, PI 698818) has introgressed segments from A. cardenasii on the top of chromosome A02, the bottom of A02, and the bottom of A03. Line S273 (TifGP-4, Reg. no. GP-245, PI 698819) has the introgressed segments on the top of A02 and the bottom of A03. The level of resistance to LLS observed in these two lines was not significantly different. Segments on the top of A02 combined with the bottom of A03 accounted for the majority of LLS resistance. Some breeders may choose to use TifGP-4 to minimize the chance of linkage drag. However, important traits may reside in the segment on the bottom of A02, so we are also releasing TifGP-3. KASPar assays can be used to determine the presence or absence of each introgressed segment, which will allow breeders to use marker-assisted selection. These two germplasm lines were jointly released by the USDA-ARS and University of Georgia in 2021.

Automated summary

Late leaf spot (LLS) disease in peanuts can be controlled through breeding resistant cultivars, with S07 and S273 being two breeding lines with high levels of resistance due to alien introgressions from A. cardenasii. These lines were developed through hybridization with 'TifNV-High O/L' and genotyped for specific introgressed segments, offering potential for marker-assisted selection in breeding programs.