Light emitting diodes optimisation for secondary metabolites production by Droseraceae plants

The most abundant active compound in Droseraceae is plumbagin, a naphthoquinone widely used for medical purposes due to its antimicrobial, antitussive, antimalarial and anticancer properties. In this work, we created a light-emitting diode (LED) based culture illumination setup as an alternative to fluorescent lamps traditionally used as a light source in plant in vitro cultures. The plants of Drosera binata and Drosera peltata cultured under LED illumination grew equally well and produced similar amounts of biologically active compounds as plants grown under fluorescent lamps. The plants were cultured on two media differing in mineral composition, sucrose content and pH. Secondary metabolites were extracted with ethanol from the plants after harvesting. The extracts were subjected to HPLC and microbiological analyses. We observed differences in morphology and secondary metabolism between plants of the same species grown on different media. However, we did not note significant changes in secondary metabolite yield under assessed lighting conditions. We propose LEDs as a more efficient, eco-friendly and economically reasonable source of light for big scale in vitro production of plumbagin in Drosera species than fluorescent lamps.

Automated summary

This study found that LED illumination is equally effective as fluorescent lamps for cultivating Drosera binata and Drosera peltata, resulting in similar production of biologically active compounds. Different media compositions may affect the morphology and secondary metabolism of the plants, but do not significantly alter the yield of secondary metabolites under assessed lighting conditions. LEDs are proposed as a more efficient, eco-friendly, and economically reasonable light source for large-scale in vitro production of plumbagin in Drosera species.