Pharmacokinetics and brain distribution studies of 6-hydroxykynurenic acid and its structural modified compounds

Objectives 6-Hydroxykynurenic acid (6-HKA) is an organic acid component in extracts of Ginkgo biloba leaves and acts as a major contributor to neurorestorative effects, while its oral bioavailability was low. Therefore, using prodrug method to improve the bioavailability and brain content of 6-HKA is significant. Methods Three structural modified compounds of 6-HKA were synthesized, and ultra performance liquid chromatography-tandem mass spectrometry methods for quantification of these structural modified compounds in rat plasma and rat brain homogenate were established and comprehensively validated. The methods were effectively applied to investigate the effects of structural modification on apparent permeability coefficients in cells, the pharmacokinetics and the brain distribution in rats. Key findings The results illustrated that esterification can greatly improve the apparent permeability coefficient and bioavailability of 6-HKA. Comparing with direct oral administration of 6-HKA, the bioavailability of isopropyl ester was greatly improved (from 3.96 1.45% to 41.8 +/- 15.3%), and the contents of 6-HKA in rat brains (49.7 +/- 9.2 ng/g brain) were significantly higher after oral administration. Conclusions The bioavailability and the brain content of 6-HKA can be improved by the prodrug method. Among three structural modified compounds, isopropyl-esterified 6-HKA was the most promising treatment.

Automated summary

6-Hydroxykynurenic acid (6-HKA) is an organic acid component in Ginkgo biloba leaf extracts that contributes to neurorestorative effects, but has low oral bioavailability. This study showed that esterification can significantly improve the apparent permeability coefficient and bioavailability of 6-HKA, with isopropyl ester being the most promising treatment.