Journal
JOURNAL OF PHARMACY AND PHARMACOLOGY
Volume 73, Issue 12, Pages 1617-1629Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jpp/rgab145
Keywords
Sophora flavescens; oxymatrine; histamine-independent itch
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Funding
- National Natural Science Foundation of China [81373993, 81861138042, 81673634, 82074137, 81703671]
- Science and Technology Planning Project of Guangdong Province [2017A020213029]
- Guangdong Basic and Applied Basic Research Foundation [2020A1515011515]
- Jinan University Graduate Study Abroad Program
- Lin Jian Biomedical Development Fund of Jinan University
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This study identified 21 compounds from Sophora flavescens, with 5 potential bioactive compounds discovered. Oxymatrine (OMT) significantly reduced scratching bouts in two histamine-independent itch models and decreased mRNA expression of transient receptor potential ankyrin 1 (TRPA1) channel. OMT also reduced scratching bouts induced by allyl isothiocyanate (AITC).
Objectives This study aimed to discover the active compounds of Sophora flavescens Ait. (SF), the anti-itch effects and underlying mechanisms of oxymatrine (OMT), one of the bioactive compounds from SF. Methods Dorsal root ganglion cell membrane immobilized chromatography was used to screen potential anti-pruritic active compounds from SF. The scratching behaviour was analysed to systematically study the anti-pruritic effects of OMT in chloroquine- (CQ), peptide Ser-Leu-Ile-Gly- Arg-Leu- (SLIGRL), histamine- (HIS) and al lyl-isothiocyanate-(AITC)-induced itch mice models. Real-time quantitative PCR, in-vivo study and molecular docking were employed to explore the underlying mechanisms. Key findings All in all, 21 compounds of SF were identified and 5 potential bioactive compounds were discovered. OMT significantly reduced scratching bouts in two HIS-independent itch models induced by CQ and SLIGRL but was not effective in the HIS-induced itch model. OMT reduced scratching bouts in a dose-dependent manner and decreased the messenger RNA (mRNA) expression of transient receptor potential ankyrin 1 (TRPA1) channel in two HIS-independent itch models; in addition, OMT reduced the wipes and scratching bouts induced by AITC. Conclusions This study discovered five potential anti-pruritic compounds including OMT in the SF extract, and OMT has strong anti-pruritic effects in HIS-independent itch via TRPA1 channel.
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