4.7 Article

Short-Term Colony-Stimulating Factor 1 Receptor Inhibition-Induced Repopulation After Stroke Assessed by Longitudinal 18F-DPA-714 PET Imaging

Journal

JOURNAL OF NUCLEAR MEDICINE
Volume 63, Issue 9, Pages 1408-1414

Publisher

SOC NUCLEAR MEDICINE INC
DOI: 10.2967/jnumed.121.263004

Keywords

Key Words; colony-stimulating factor 1 receptor; microglia; stroke; 18F-DPA-714; repopulation

Funding

  1. Horizon 2020 Programme [675417]
  2. DFG, Munster, Germany [EXC1003]
  3. Interdisciplinary Center for Clinical Research (IZKF core unit PIX), Munster, Germany
  4. Herbert Worch Foundation, Bonn, Germany

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This study investigated the effects of microglia repopulation on inflammation and functional outcomes in an ischemic mouse model. The results showed that microglia repopulation can resolve the inflammatory imbalance and contribute to functional recovery after ischemia.
Studies on colony-stimulating factor 1 receptor (CSF-1R) inhibition & ndash; induced microglia depletion indicated that inhibitor withdrawal allowed the renewal of the microglia compartment via repopulation and resolved the inflammatory imbalance. Therefore, we investigated for the first time (to our knowledge) the effects of microglia repopula-tion on inflammation and functional outcomes in an ischemic mouse model using translocator protein (TSPO)-PET/CT and MR imaging, ex vivo characterization, and behavioral tests. Methods: Eight C57BL/6 mice per group underwent a 30-min transient occlusion of the middle cerebral artery. The treatment group received CSF-1R inhibitor in 1,200 ppm PLX5622 chow (Plexxikon Inc.) from days 3 to 7 to induce microglia/macrophage depletion and then went back to a con-trol diet to allow repopulation. The mice underwent T2-weighted MRI on day 1 after ischemia and 18F-labeled N,N-diethyl-2-(2-[4-(2-fluo roethoxy)phenyl]-5,7-dimethylpyrazolo[1,5-alpha]pyrimidine-3-yl)aceta mide (F-18-DPA-714) (TSPO) PET/CT on days 7, 14, 21, and 30. The percentage injected tracer dose per milliliter within the infarct, con-tralateral striatum, and spleen was assessed. Behavioral tests were performed to assess motor function recovery. Brains were har-vested on days 14 and 35 after ischemia for ex vivo analyses (immu-noreactivity and real-time quantitative polymerase chain reaction) of microglia-and macrophage-related markers. Results: Repopula-tion significantly increased 18F-DPA-714 uptake within the infarct on days 14 (P < lt; 0.001) and 21 (P = 0.002) after ischemia. On day 14, the ionized calcium binding adaptor molecule 1 (Iba-1)& ndash;positive cell population showed significantly higher expression of TSPO, CSF-1R, and CD68, in line with microglia repopulation. Gene expression analyses on day 14 indicated a significant increase in microglia-related markers (csf-1r, aif1, and p2ry12) with repopulation, whereas peripheral cell recruitment & ndash;related gene expression decreased (cx3cr1 and ccr2), indicative of peripheral recruitment during CSF-1R inhibition. Similarly, uncorrected spleen uptake was significantly higher on day 7 after ischemia with treatment (P = 0.001) and decreased after drug withdrawal. PLX5622-treated mice walked a longer distance (P < 0.001) and more quickly (P = 0.009), and showed greater forelimb strength (P < 0.001), than control mice on day 14. Conclusion: This study highlighted the potential of 18F-DPA-714 PET/ CT imaging to track microglia and macrophage repopulation after short-term CSF-1R inhibition in stroke.

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