Ipsilateral Stimulus Encoding in Primary and Secondary Somatosensory Cortex of Awake Mice

Lateralization is a hallmark of somatosensory processing in the mammalian brain. However, in addition to their contralateral representation, unilateral tactile stimuli also modulate neuronal activity in somatosensory cortices of the ipsilateral hemisphere. The cellular organization and functional role of these ipsilateral stimulus responses in awake somatosensory cortices, especially regarding stimulus coding, are unknown. Here, we targeted silicon probe recordings to the vibrissa region of primary (S1) and secondary (S2) somatosensory cortex of awake head-fixed mice of either sex while delivering ipsilateral and contralateral whisker stimuli. Ipsilateral stimuli drove larger and more reliable responses in S2 than in S1, and activated a larger fraction of stimulus-responsive neurons. Ipsilateral stimulus-responsive neurons were rare in layer 4 of S1, but were located in equal proportion across all layers in S2. Linear classifier analyses further revealed that decoding of the ipsilateral stimulus was more accurate in S2 than S1, whereas S1 decoded contralateral stimuli most accurately. These results reveal substantial encoding of ipsilateral stimuli in S1 and especially S2, consistent with the hypothesis that higher cortical areas may integrate tactile inputs across larger portions of space, spanning both sides of the body.

Automated summary

Lateralization is a characteristic of somatosensory processing in the mammalian brain. This study reveals that ipsilateral tactile stimuli drive larger and more reliable responses in the secondary somatosensory cortex (S2) compared to the primary somatosensory cortex (S1). The cellular organization and functional role of these ipsilateral stimulus responses differ between S1 and S2, suggesting that higher cortical areas may integrate tactile inputs across larger portions of space.