4.4 Review

Lateral flow immunoassay for small-molecules detection in phytoproducts: a review

Journal

JOURNAL OF NATURAL MEDICINES
Volume 76, Issue 3, Pages 521-545

Publisher

SPRINGER JAPAN KK
DOI: 10.1007/s11418-022-01605-6

Keywords

Immunoassay; Immunochromatographic strip test; Lateral flow immunoassay; Plant secondary metabolites; Hapten; Small molecules

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Phytoproducts play important roles in various industries, and small-molecule organic compounds can be used to determine the quality and safety of plant samples. The lateral flow immunoassay (LFA) is a simple, portable, and rapid method for detecting and analyzing phytoproducts.
Phytoproducts are involved in various fields of industry. Small-molecule (Mw < 900 Da) organic compounds can be used to indicate the quality of plant samples in the perspective of efficacy by measuring the necessary secondary metabolites and in the perspective of safety by measuring the adulterant level of toxic compounds. The development of reliable detection methods for these compounds in such a complicated matrix is challenging. The lateral flow immunoassay (LFA) is one of the immunoassays well-known for its simplicity, portability, and rapidity. In this review, the general principle, components, format, and application of the LFA for phytoproducts are discussed.

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