Journal
JOURNAL OF MOLECULAR NEUROSCIENCE
Volume 72, Issue 4, Pages 759-771Publisher
SPRINGERNATURE
DOI: 10.1007/s12031-021-01949-w
Keywords
Traumatic brain injury; Proteome; Phosphoproteome; Protein-protein interaction; Cross-talk pathway and process
Categories
Funding
- National Natural Science Foundation of China [81771322, 82171363, 82171321, 81871023]
- Youth Talent Lifting Project [17-JCJQQT-037]
- Youth Nova Program of Shanxi [2021KJXX-19]
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In this study, differentially expressed proteins (DEPs) and differentially expressed phosphoproteins (DEPPs) in the early and late stages of TBI were identified using TMT labeling, Phos affinity enrichment, and high-resolution LC-MS/MS analysis. Integrative analyses revealed the different and similar pathophysiologic mechanisms between the early and late stages, providing insights into useful biomarkers and underlying mechanisms in TBI and its sequelae.
Traumatic brain injury (TBI) is a major public health concern all around the world. Accumulating evidence suggests that pathological processes after brain injury continuously evolve. Here, we identified the differentially expressed proteins (DEPs) and differentially expressed phosphoproteins (DEPPs) in the early and late stages of TBI in mice using TMT labeling, enrichment of Phos affinity followed, and high-resolution LC-MS/MS analysis. Subsequently, integrative analyses, including functional enrichment-based clustering analysis, motif analysis, cross-talk pathway/process enrichment analysis, and protein-protein interaction enrichment analysis were performed to further identify the different and similar pathophysiologic mechanisms in the early and late stage. Our work reveals a map of early and late-stage protein networks in TBI, which shed light on useful biomarkers and the underlying mechanisms in TBI and its sequelae.
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