Detection of Norovirus RNA based on catalytic hairpin assembly and magnetic separation of DNA AgNCs

Based on catalytic hairpin assembly (CHA) and magnetic separation, a fluorescence biosensor of Norovirus RNA was developed. In this detection strategy, these two isothermal signal amplification techniques as well as brightly fluorescent DNA-templated silver nanoclusters (AgNCs) of high quantum yield were employed, with the aim of highly sensitive detection of Norovirus RNA at room temperature. Results showed that the designed strategy of sensor could be successfully established and implemented. Under optimal reaction conditions, this sensor showed a good linear response towards the concentrations of target RNA in the range of 1.0-100 nM with a detection limit of 0.82 nM. A quenching phenomenon was found in this sensoring system, which was contributed together by the biotin-avidin complex and magnetic nanoparticles. The phenomenon was confirmed and explained for reference value of AgNCs based biosensors. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

A fluorescence biosensor for Norovirus RNA was developed using catalytic hairpin assembly (CHA) and magnetic separation techniques, with the aim of highly sensitive detection at room temperature. The sensor showed a good linear response towards target RNA concentrations in the range of 1.0-100 nM with a detection limit of 0.82 nM. A quenching phenomenon observed in the sensor system was attributed to the interaction between the biotin-avidin complex and magnetic nanoparticles.