Journal
JOURNAL OF MOLECULAR LIQUIDS
Volume 340, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.molliq.2021.116895
Keywords
Phytochromes; PhyB; Citrus; Structural modeling; MD simulations
Funding
- National Key Research and Development Program of China [2018YFD1000101]
- Hubei Provincial Natural Science Foundation of China [2019CFA014]
- Starting Research Grant for High-level Talents from Guangxi University
- Postdoctoral Project from Guangxi University
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This study explored the structure and function of Phytochromes in citrus, comparing them with those in Arabidopsis and soybean, revealing significant structural differences. Molecular dynamics simulation studies suggested that the GAF domain of citrus Phytochrome may contribute to the functional variations observed.
Phytochromes (Phys) are known as red/far-red light photoreceptors and are responsible for directing the photosensory responses across the species, majorly from fungal, bacterial and plant kingdoms. Such responses majorly include photosynthetic potential and pigmentation in bacteria, whereas in a plant, they are involved in chloroplast development and photomorphogenesis. Many prokaryotic Phys have been modelled for their structural and functional analysis, but their plant counterparts have not been explored yet. To date, only the crystal structures of the photo-sensing module (PSM) of PhyB isoform from Arabidopsis thaliana and Glycine max have been resolved experimentally. Thus, in this study, we elucidated the complete 3D structure of Citrus PhyB. Initially, the structure and organization of the Citrus PhyB have been predicted computationally, which were found to have the same domain organization as A. thaliana and G. max PhyBs, yet their considerable distinct structural difference indicated potential divergence in signaling and functioning. Therefore, to evaluate the structural implications of Citrus PhyB, we compared its structure with A. thaliana and G. max PhyBs using molecular dynamics (MD) simulation approaches. The modeling studies revealed that the region of Citrus PhyB-GAF domain possibly contributes to the variations between Citrus, A. thaliana and G. max PhyBs structures. Hence, structural and molecular insights into Citrus PhyB can help to understand better the Phys and thus, an essential framework can be designed for optogenetic reagents and various agricultural/horticulture benefits. (C) 2021 Elsevier B.V. All rights reserved.
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