4.3 Article

Method for high-throughput antifungal activity screening of bacterial strain libraries

Journal

JOURNAL OF MICROBIOLOGICAL METHODS
Volume 189, Issue -, Pages -

Publisher

ELSEVIER
DOI: 10.1016/j.mimet.2021.106311

Keywords

Antagonism; Coculture; Biocontrol; Antibiosis

Funding

  1. U.S. Department of Agriculture Agricultural Research Service CRIS project [2030-42000-054-00D]

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Our laboratory has developed a high-throughput screening method for antifungal phenotypes, which involves transferring bacterial libraries in 96 well format to agar pour plates inoculated with spores of the target fungus. This method improves upon traditional bacterial/fungal coculture plate methods by measuring antifungal activity as inhibition of radial fungal growth.
Our laboratory developed a high-throughput screen for antifungal phenotypes in which bacterial libraries in 96 well format are transferred to agar pour plates inoculated with spores of the target fungus. This method is an improvement over bacterial/fungal coculture plate methods that measure antifungal activity as inhibition of radial fungal growth.

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