Better detection of Torque teno virus in children with leukemia by metagenomic sequencing than by quantitative PCR

Torque teno virus (TTV) is a group of chronically persisting viruses with a short circular DNA genome. TTV demonstrates a wide sequence diversity and a large majority of humans are chronically infected by one or more types of TTV. As TTV is ubiquitous, and viral replication correlates with immune status, TTV has been studied as a marker to assess global functional immune competence in transplant recipients. Most studies of the prevalence, amounts, and variation in TTV have been performed using PCR assays. We here present a comparison of the most frequently used quantitative PCR (qPCR) assay for TTV with shotgun metagenomic sequencing for detection and characterization of TTV in a cohort of pediatric cancer patients. The results show that TTV is more common than the qPCR assays indicate, and analysis of the TTV genome sequences indicate that a qPCR with primers and probe designed on a conserved region of the TTV genome may fail to detect some of the TTV strains found in this study.

Automated summary

TTV is a group of chronically persisting viruses with a wide sequence diversity, and studies suggest that it is more common than detected by conventional qPCR assays. Analysis of TTV genome sequences reveals that some strains may not be detected by qPCR designed on a conserved region of the TTV genome.