Antibacterial properties of silver nanoparticles synthesized via nanosecond pulsed laser ablation in water

Ag nanoparticles were synthesized in pure water by a nanosecond pulsed laser ablation process and characterized using transmission electron microscopy and ultraviolet-visible spectroscopy. The as-synthesized nanoparticles were determined to have an average particle size of 10.17 & PLUSMN; 2.95 nm. Their absorption spectra were consistent with that of Ag nanoparticles produced through chemical methods. The antibacterial properties of Ag nanoparticles at concentrations of 100 and 250 mu g/ml were studied against the gram-positive bacterium Staphylococcus aureus at two infectious inocula (10(3) and 10(5) CFU/ml). Over a 24-h test period, the samples containing 100 mu g/ml exhibited early bacteriostatic activity but did not stop long-term bacterial growth, while the 250 mu g/ml samples demonstrated bactericidal and bacteriostatic effects. These results highlight the use of Ag nanoparticles synthesized through laser ablation as green and effective antibacterial agents for biomedical applications.

Automated summary

Ag nanoparticles were synthesized using a nanosecond pulsed laser ablation process, and their antibacterial properties were studied, showing potential for biomedical applications.