Journal
JOURNAL OF INFECTIOUS DISEASES
Volume 225, Issue 5, Pages 846-855Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jiab496
Keywords
Chlamydia; genital tract; antibody; serology; endometrium; immunoproteome
Categories
Funding
- National Institute of Allergy and Infectious Diseases [R01AI119164, U19AI084024, U19AI144181]
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This study found that protein-specific C. trachomatis antibodies are not sufficient to protect against ascending or incident infection. However, cervical C. trachomatis gene transcript abundance positively correlates with C. trachomatis protein immunogenicity. These abundant and broadly recognized antigens are viable vaccine candidates.
Background Previous research revealed antibodies targeting Chlamydia trachomatis elementary bodies was not associated with reduced endometrial or incident infection in C. trachomatis-exposed women. However, data on the role of C. trachomatis protein-specific antibodies in protection are limited. Methods A whole-proteome C. trachomatis array screening serum pools from C. trachomatis-exposed women identified 121 immunoprevalent proteins. Individual serum samples were probed using a focused array. Immunoglobulin (Ig) G antibody frequencies and endometrial or incident infection relationships were examined using Wilcoxon rank sum test. The impact of the breadth and magnitude of protein-specific IgGs on ascension and incident infection were examined using multivariable stepwise logistic regression. Complementary RNA sequencing quantified C. trachomatis gene transcripts in cervical swab samples from infected women. Results IgG to pGP3 and CT_005 were associated with reduced endometrial infection; anti-CT_443, anti-CT_486, and anti-CT_123 were associated with increased incident infection. Increased breadth of protein recognition did not however predict protection from endometrial or incident infection. Messenger RNAs for immunoprevalent C. trachomatis proteins were highly abundant in the cervix. Conclusions Protein-specific C. trachomatis antibodies are not sufficient to protect against ascending or incident infection. However, cervical C. trachomatis gene transcript abundance positively correlates with C. trachomatis protein immunogenicity. These abundant and broadly recognized antigens are viable vaccine candidates. Protein-specific C. trachomatisantibodies are not sufficient to protect against ascending or incident infection. However, cervical C. trachomatisgene transcript abundance positively correlates with C. trachomatisprotein immunogenicity. These abundant and broadly recognized antigens are viable vaccine candidates.
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