Metagenomic sequencing with spiked-in internal control to monitor cellularity and diagnosis of pneumonia

Article Biotechnology & Applied Microbiology

A proof-of-concept study of an automated solution for clinical metagenomic next-generation sequencing

Y. Luan et al.

Summary: The study introduced a device called NGSmaster that automates the wet-lab workflow of metagenomic next-generation sequencing (mNGS), reducing hands-on time and microbial contaminations significantly. The results showed that NGSmaster can meet the microbiological diagnostic needs in various sample types and shorten the turnaround time for testing results.

JOURNAL OF APPLIED MICROBIOLOGY (2021)

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Article Infectious Diseases

Metagenomic next-generation sequencing for the diagnosis of suspected pneumonia in immunocompromised patients

Jin-Min Peng et al.

Summary: The study evaluated the potential of mNGS for immunocompromised patients with suspected pneumonia and found that while mNGS and CMTs had similar diagnostic accuracy for bacterial and viral infections, mNGS had lower accuracy for fungal infections. The combination of mNGS and CMTs may be the best diagnostic strategy.

JOURNAL OF INFECTION (2021)

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Article Biochemistry & Molecular Biology

Rapid pathogen detection by metagenomic next-generation sequencing of infected body fluids

Wei Gu et al.

Summary: The developed mNGS test using cell-free DNA from body fluids demonstrates high sensitivity and specificity for identifying pathogens, showing potential clinical utility. This method shows promise in rapid pathogen detection and may accelerate clinical decisions by providing high-specificity, unbiased detection from diverse body fluids using metagenomic sequencing.

NATURE MEDICINE (2021)

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Article Biotechnology & Applied Microbiology

Nanopore metagenomics enables rapid clinical diagnosis of bacterial lower respiratory infection

Themoula Charalampous et al.

NATURE BIOTECHNOLOGY (2019)

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Article Medical Laboratory Technology

Validation of Metagenomic Next-Generation Sequencing Tests for Universal Pathogen Detection

Robert Schlaberg et al.

ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE (2017)

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Metagenomic sequencing with spiked-in internal control to monitor cellularity and diagnosis of pneumonia

Related references

A proof-of-concept study of an automated solution for clinical metagenomic next-generation sequencing

Metagenomic next-generation sequencing for the diagnosis of suspected pneumonia in immunocompromised patients

Rapid pathogen detection by metagenomic next-generation sequencing of infected body fluids

Nanopore metagenomics enables rapid clinical diagnosis of bacterial lower respiratory infection

Validation of Metagenomic Next-Generation Sequencing Tests for Universal Pathogen Detection

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