Journal
JOURNAL OF HETEROCYCLIC CHEMISTRY
Volume 59, Issue 7, Pages 1144-1159Publisher
WILEY
DOI: 10.1002/jhet.4454
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Funding
- Shiraz University of Medical Sciences [95-01-36-13586]
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Thirteen novel Schiff base derivatives were synthesized and their cytotoxic activities against tumor cells and normal cells were evaluated. One of the compounds, M-12, showed high activity and was found to bind to VEGFR-2 and JNK3-MAP kinase. Based on the in vitro experiments and in silico predictions, M-12 could be a promising candidate for future anticancer studies.
Thirteen novel isatin-ampyrone Schiff bases derivatives were synthesized in only two steps of 70%-90% overall yields. In vitro cytotoxic activity of these new Schiff bases against three human tumor cell lines (MCF-7, A549, and SCOV3) as well as normal breast cell line (MCF-10A) were evaluated by MTT assay. Structure-activity relationship of the tested compounds revealed that chlorine group at C-5 position of the isatin ring significantly increased the cytotoxic activity. This study generally led to introduce a highly active molecule (M-12) with IC50 values of 5.12, 25.5, and 12.9 mu M, on MCF-7, A549, and SCOV3, respectively. Furthermore, molecular docking studies of the synthesized compounds were also done to investigate their binding modes towards VEGFR-2 and JNK3-MAP kinase as the main targets for isatin-containing anticancer agents. Binding free energy values of the compounds showed positive correlation with their cytotoxic activities. To confirm the docking results, molecular docking simulations of potent compound (M-12) against VEGFR-2 and JNK3 MAP kinase were also performed. According to the cytotoxic results and in silico ADMET predictions together, M-12 can be considered as a potent candidate for the future anticancer studies.
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